It took Shinji only a few minutes to complete all the preparations for the surgery. He activated the cryogenic canister and took out a small clump of First's cells from inside.
Shinji had secretly obtained this small clump of First's cells through Yakumo.
After First passed away, Second used the most advanced techniques available to preserve a large quantity of First's cells. This sample was one of those Second had preserved at the time.
Because these cells had been taken directly from First's body, rather than cultivated in a laboratory, they were exceptionally active. The Yang Release Chakra they contained was also closer in strength to what First had possessed while alive.
Of course,
that wasn't what Shinji cared about. What mattered to him was the chakra of Ōtsutsuki Ashura still lingering in this clump of cells taken directly from First's body.
Going by Madara Uchiha's successful case,
the chakra of Ōtsutsuki Ashura contained in a small amount of flesh taken directly from First's body was enough to achieve Yin-Yang Release and awaken a sufficient amount of the Power of All Things, thereby unlocking the Rinnegan.
After all, what was truly needed to awaken the Rinnegan was the Power of All Things produced when high-quality Yin Release Chakra and Yang Release Chakra clashed.
Quality was what mattered!
Shinji raised the scalpel. "Let's begin!"
"Okay!"
Karin nodded.
"Remember, you have to use your Dojutsu to neutralize the Yang Release Chakra in First's cells. Your Eternal Mangekyo Sharingan is the key to whether this surgery succeeds!"
After giving that final reminder, Shinji said no more. He immediately opened another transplant site on Karin's arm.
Implanting the First cells
Stitching up the transplant site.
Injecting the Fusion Agent.
Shinji completed the first half of the surgery in one smooth sequence, taking only a